Influences of ionic liquids on morphology and activity of lysozyme crystal

化学工业与工程

Chemcial Industry and Engineering

2023, Vol. 40

Issue (1) :112-119 DOI: 10.13353/j.issn.1004.9533.20220364

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Influences of ionic liquids on morphology and activity of lysozyme crystal

LI Na, WANG Zhanzhong

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract In this study, egg white lysozyme was used as a model protein, crystals were prepared by cooling crystallization and the activity of lysozyme crystal was determined by the turbidity method, and changes in morphology and activity of lysozyme crystals were investigated under the condition of adding four kinds of hydrophilic ionic liquids (ILs) respectively. The four ILs are 1-butyl-3 methylimidazolium tetrafluoroborate ([C₄mim]BF₄), 1-butyl-3 methylimidazolium chloride ([C₄mim]Cl), 1-butyl-3 methylimidazolium bromide ([C₄mim]Br) and 1,3 dimethylimidazolium iodide ([dmim]I). The crystal morphology of the lysozyme during the crystallization process was analyzed by optical microscopy, and the results showed that the addition of IL affects the morphology and size of the lysozyme crystal.[C₄mim]BF₄ mediates lysozyme to form rod-like crystals. Both[C₄mim]Cl and[C₄mim]Br do not change the morphology of lysozyme crystals but promote the growth of lysozyme crystals compared with no IL addition. In the presence of[C₄mim]Cl and[C₄mim]Br, lysozyme crystals are square and increase in size. When[dmim]I was added, lysozyme shows needle-like crystals. The activity of the lysozyme crystal was determined by turbidimetry. The results show that different ILs have different effects on the activity of lysozyme crystals. Among them, the addition of[C₄mim]BF₄ and[dmim]I decreased the activity of lysozyme crystals, while the addition of[C₄mim]Cl and[C₄mim]Br increased the activity of enzyme crystals.

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	LI Na
	WANG Zhanzhong

Keywords： lysozyme ionic liquid crystallization enzymatic activity

Received 2022-07-23;

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LI Na, WANG Zhanzhong.Influences of ionic liquids on morphology and activity of lysozyme crystal[J] Chemcial Industry and Engineering, 2023,V40(1): 112-119

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