Preparation of Immobilized Marine Petroleum-Degrading Bacteria and its Degradation Characteristics or Petroleum-Containing Seawater

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Abstract In this work, a marine petroleum-degrading bacteria (SI-JHS) which was insolated previously in our laboratory, was immobilized at different embedding conditions to increase the degradation efficiency for petroleum-containing seawater. The effects of environmental conditions in seawater such as pH, salinity and temperature on degradation characteristics of immobilized beads were investigated. Immobilized beads prepared by 10% polyvinyl alcohol (PVA) and 1% sodium alginate (SA) as embedding material, and saturated boric acid solutions containing 2% calcium chloride (CaCl₂) as crosslinker. The better mass transfer performance and greater mechanical strength of immobilized beads were obtained when bacteria entrapment reached 20% and 5% activated carbon was added. The best degradation conditions for immobilized beads to degrade petroleum-containing seawater were:pH at 7.0~7.5, salinity at 3%~4% and temperature at 30~35℃. The degradation rate of petroleum by SI-JHS-immobilized beads was 97.8%, which was 22.6% higher than free bacteria. The degradation process could be followed by the pseudo first order dynamic model.

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	Articles by authors
	Jiang Tianxiang
	Zhang Aijun
	Wang Wenhua
	Wang Jing
	Ren Huafeng

Keywords： marine petroleum-degrading bacteria； immobilized beads； petroleum-containing seawater； degradation efficiency

Received 2017-09-28;

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Jiang Tianxiang, Zhang Aijun, Wang Wenhua, Wang Jing, Ren Huafeng.Preparation of Immobilized Marine Petroleum-Degrading Bacteria and its Degradation Characteristics or Petroleum-Containing Seawater[J] Chemcial Industry and Engineering, 2019,V36(3): 35-41

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