Study on the process of macroporous resin-C18 column separation and purification of <em>β</em>-caryophyllene and its antioxidation properties

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Abstract The crude extract of β-caryophyllene was obtained from the transgenic microalgae culture solution by reduced pressure concentration combined with ethanol extraction process, and the products were further concentrated and purified by establishing a macroporous resin combined with C18 spherical column separation method. Six different types of macroporous resin were investigated, and HPD-100 macroporous resin was selected as the optimal adsorbent for β-caryophyllene. The separation and purification conditions of crude extract were optimized respectively under static and dynamic adsorption-desorption conditions. Through the optimization of temperature, pH, time and desorption agent during static adsorption and desorption, the static adsorption and desorption rates reached 87.8% and 80.8% respectively. In the dynamic adsorption and desorption experiment, the initial loading flow rate was 2 BV·h^-1, the loading volume was 28 BV, the adsorption rate was 98.0%. The desorption rate reached 64.8% using ethanol as eluent at a flow rate of 1.5 mL·min^-1 with the elution volume of 7 BV. Subsequently, 5 mL eluate was loaded to a C18 column with methanol as the mobile phase and the elution flow rate of 1.0 mL·min^-1. The purity of β-caryophyllene in the collected sample reached 99.0% which increased 63.2% compared with the crude extract, indicating the efficiency of this method. In vivo and in vitro antioxidant capacity tests were conducted using the purified β-caryophyllene. The clearance capacity for DPPH, ABTS, and ·O^-₂ was 82.2%, 76.7%, and 82.4%, respectively, at 1 000 μmol·L^-1 and 40.75% reduction in PC12 cells at the addition of 10 μmol·L^-1. Moreover, molecular docking simulations indicated that β-caryophyllene had excellent affinity to SOD, CAT, POD and GPX.

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	HE Weigang
	SUN Chengjun
	Lü Shijun
	TAO Yuheng
	YAN Shenghu
	WANG Liqun
	QING Qing

Keywords： β-caryophyllene macroporous resin adsorption separation and purification antioxidant

Received 2023-03-07;

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HE Weigang, SUN Chengjun, Lü Shijun, TAO Yuheng, YAN Shenghu, WANG Liqun, QING Qing.Study on the process of macroporous resin-C18 column separation and purification of β-caryophyllene and its antioxidation properties[J] Chemcial Industry and Engineering, 2025,V42(2): 35-44

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