Effect of drug loading on the formation of insulin-contained W/O microemulsion

化学工业与工程

Chemcial Industry and Engineering

2025, Vol. 42

Issue (1) :47-53 DOI: 10.13353/j.issn.1004.9533.20230503

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Effect of drug loading on the formation of insulin-contained W/O microemulsion

YANG Xu¹, YU Zhanfeng², WEI Zhenping¹

1. School of Chemical Engineering, Tianjin University, Tianjin 300350, China;
2. Shenzhen Taitai Pharmaceutical Industry Co. Ltd., Shenzhen 518057, Guangdong, China

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Abstract The advantages of microemulsion as an oral insulin carrier are its high dispersibility and retention of insulin biological activity, which benefit insulin intestinal absorption. Traditional formulation screening method of insulin-contained microemulsion (ICME) usually based on the addition of insulin to the insulin-free microemulsion (IFME) after IFME formulation is determined by pseudo-ternary phase diagram. However, as a biomacromolecule, insulin has effect on oil-water interface tension and structure, which means the pseudo-ternary phase diagram will change after adding insulin. In such situation, the stability of ICME will be impacted. In this study, pseudo-ternary phase diagrams of IFME and ICME were compared and the optimized formulation of ICME was determined. Results showed that the addition of insulin changed the microemulsion area in pseudo-ternary phase diagram and the maximum range is 56.1%. Based on ICME pseudo-ternary phase diagram, the optimal formulation of ICME was determined as Span-80 22.6%, Tween-80 9.7%, ethanol 8.1%, isopropyl myristate (IPM) 40.4% and aqueous phase 19.2%(mass fraction), with a diameter of 84.25 nm. The result in this experiment is valuable for the formulation screening of drug-contained microemulsion.

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	YANG Xu
	YU Zhanfeng
	WEI Zhenping

Keywords： microemulsion insulin formulation screening pseudo-ternary phase diagram

Received 2023-05-06;

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YANG Xu, YU Zhanfeng, WEI Zhenping.Effect of drug loading on the formation of insulin-contained W/O microemulsion[J] Chemcial Industry and Engineering, 2025,V42(1): 47-53

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