Abstract In this paper,a purification route using the liquid chromatogram on a loboratory scale was established on thymosin α1 synthesized by the solid-phase synthetic method using Fmoc group fo protecting α-amino acid.Two steps were included in the purification route.First was the Ion-exchange chromatography (IEC) using DEAE Sepharose Fast Flow as the matrix.After this step,majority of impure peptides was removed.Second was the preparation RP-HPLC on C18 column.Then the final product's purity attained to 95% and the values of the biological activity all meet the requirements.Those operation conditions during the separation and purification process were also studied and optimized in this thesis.
HUANG He,WAN Li-peng,WU Lei,GAN Yi-ru .Study on the Separation and Purification Techniques of Solid-phase Synthetic Shymosin α1 by the Liquid Chromatogram[J] Chemcial Industry and Engineering, 2001,V18(6): 331-335
2001, Vol. 18 
