Kinetics of Esterification Reactions Catalyzed by Surfactant-Coated Lipase in Organic Solvents

Related Articles

Abstract The lipase from Candida rugosa was coated with synthetic didodecanyl glutamate ribitol and used to catalyze esterification of lauric acid and lauryl alcohol in organic solvents.The optimal reaction conditions were determined,which are buffer pH for lipase coating,6 8;reaction temperature,30℃;solvent,isooctane.The kinetics of the esterification reaction obeyed the Ping-Pang Bi-Bi mechanism with an inhibition by lauryl alcohol.The kinetic parameters under the optimal conditions were determined.The Michaelis constants of lauric acid and lauryl alcohol were 29 1 and 71 4mmol·L -1 ,respectively.The maximum rate was 0 66mmol·min -1 ·g -1 .The inhibition constant of lauryl alcohol was 11 3mmol·L -1 .The results predicted by the kinetic model were in good agreement with experiment.

	Service

	Email this article
	Add to my bookshelf
	Add to citation manager
	Email Alert
	RSS
	Articles by authors

Keywords：

Received 2002-11-15; published 2002-11-15

Cite this article:

DING Hui,XING Ai-hua,SONG Bao-dong,WU Jin-chuan .Kinetics of Esterification Reactions Catalyzed by Surfactant-Coated Lipase in Organic Solvents[J] Chemcial Industry and Engineering, 2002,V19(6): 417-421

[1]	ZAKSA ,KLIBABOVAM .Enzymescatalysisinorganicmediaat100℃[J].Science.1984,224(4654):1249-1253
[2]	GOTOM ,KAMIYAN ,NAKASHIOF .Enzymaticinteresterificationoftriglyceridewithsurfactant-coatedlipaseinorganicmedia[J].BiotechnolBioeng,1995,45(1):27-32.
[3]	DORDICKJS .Enzymaticcatalysisinmonophasicorganicsolvents[J].EnzymeMicrobTechnol,1989,11:194-211.
[4]	GOTOM ,KAMEYAMAH .Designofsurfactantssuitableforsurfactant-coatedenzymesascatalystsinorganicmedia[J].JChemEngJpn,1993,26(1):109-111.
[5]	OKHATAY ,FUJIMOTOY ,IJIROKA .Alipid-coatedlipaseasanenantioselectiveestersyn thesiscatalystinhomogeneousorganicsolvents[J].JOrgChem,1995,60:2244-2250.
[6]	OKAHATAY ,IJIROKA .Alipid-coatedlipaseasanewcatalystfortriglyceridesynthesisinor ganicsolvents[J].JChemSocChemCommun,1988,(20):1392-1394.
[7]	KAMIYAN ,GOTOM .Howisenzymaticselec tivityofmentholesterificationcatalyzedbysurfac tant-coatedlipasedeterminedinorganicmedia[J].BiotechnolProg,1997,13(4),488-492.
[8]	GOTOM ,KAMIYAN .Enzymaticesterificationbysurfactant-coatedlipaseinorganicmedia[J].BiotechnolProg,1994,10(3):263-268.
[9]	HUANGSHIH -YOW ,CHANGHSIAO -LI.Ki neticstudyontheesterificationofgeraniolandaceticacidinorganicsolventsusingsurfactant-coatedlipase[J].JChemTechnolBiotechnol,1999,74:183-187.
[10]	GOTOM ,MATSUMOTOM ,KONDOK .Devel opmentofnewsurfactantforliquidsurfactantmem braneprocess[J].JChemEngJpn,1987,20(2):157-164.
[11]	宋宝东、邢爱华、吴金川.谷氨酸二烷酯核糖醇的合成及应用[J].石油化工,2001,30(增):376-378.
[12]	KAMIYAN ,GOTOM .Effectofusingaco-sol ventinthepreparationofsurfactant-coatedlipas esoncatalyticactivityinorganicmedia[J].JFer mentBioeng,1996,82:37-41.
[13]	HAND ,RHEEJS .Characteristicsoflipase-catalyzedhydrolysisofoliveoilinATO -isooctanereversemicelles[J].BiotechnolBioeng,1986,28(8):1250-1255.
[14]	KLIBANOVAM .Enzymesthatworkinorganicsolvents[J].ChemTech,1986,16(6):354-359.