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Chemcial Industry and Engineering 2006, Vol. 23 Issue (5) :403-406    DOI:
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Synthesis and Characterization of 2��,7��-Dichloride-5(6)-Fluorescein Isothiocyanate
PAN Hui-ying,GE Feng-yan,CHEN Li-gong(College of Pharmaceuticals and Biotechnology,Tianjin University,Tianjin 300072,China)
(College of Pharmaceuticals and Biotechnology,Tianjin University,Tianjin 300072,China

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Abstract Fluorescein isothiocyanate(FITC) is an important fluorescence probe.In this paper 2��,7��-dichloride-5(6)-fluorescein isothiocyanate was obtained from 4-nitrophthalic acid,4-chloride-resoreinol and zinc chloride through condensation,reduction and isothiocyanatation in a total yield of 34.78%.The structure of the products were characterized by()~1H NMR,()~(13)C NMR,IR and MS,and the fluorescent properties of the products were determined.The results showed the fluorescent properties of the products were prior to that of FITC.
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Received 2006-09-15; published 2006-09-15
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PAN Hui-ying,GE Feng-yan,CHEN Li-gong.Synthesis and Characterization of 2��,7��-Dichloride-5(6)-Fluorescein Isothiocyanate[J]  Chemcial Industry and Engineering, 2006,V23(5): 403-406
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