Preparation of Macroporous Agarose-Based Chromatographic Media Using a Double Emulsification Method and Its Binding with HBsAg

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Abstract The macroporous chromatographic media are of great importance in the field of bioseparation, especially for large biomolecules. Limited by both of the material properties and preparation technology, the macroporous chromatographic media were mostly prepared from synthetic polymers, and native polymers, e.g., agarose, were rarely used. In this paper, macroporous agarose microspheres were prepared by double emulsification method. The effects of preparation conditions on microsphere morphology and pore formation, as well as particle size and distribution were studied. It was found that the microspheres have micro-scale super-large pore structure and the pore structure shows certain regularity due to the influence of preparation conditions. The concentration of agarose solution, the ratio of internal/external oil phase to water, the speed of colostrum and the speed of ball formation are all important for pore formation. Confocal laser scanning microscopy results showed that the macroporous agarose microspheres after being functionalized with ion-exchange groups had a good binding ability to Hepatitis B Surface antigen (HBsAg) and this antigen can completely enter the inside of the microspheres. Compared with conventional agarose-based chromatographic media, macroporous agarose-based media combined macromolecular vaccine more quickly and fully, and had wide prospects of application in the field of purification of large biomolecules.

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	Articles by authors
	Huang Lan
	Huang Yongdong
	Zhao Lan
	Zhu Kai
	Wu Xuexing
	Zhou Danni
	Su Zhiguo
	Ma Guanghui
	Wang Qibao

Keywords： macroporous agarose-based microspheres； double emulsification method； pore-forming； ion-exchange chromatographic media； HBsAg

Received 2019-02-28;

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Huang Lan, Huang Yongdong, Zhao Lan, Zhu Kai, Wu Xuexing, Zhou Danni, Su Zhiguo, Ma Guanghui, Wang Qibao.Preparation of Macroporous Agarose-Based Chromatographic Media Using a Double Emulsification Method and Its Binding with HBsAg[J] Chemcial Industry and Engineering, 2019,V36(4): 70-79

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