Determination of Dimethyl Oxalate and Oxalic Acid by Reversed-Phase High Performance Liquid Chromatography

化学工业与工程

Chemcial Industry and Engineering

2016, Vol. 33

Issue (2) :91-95 DOI: 10.13353/j.issn.1004.9533.20141058

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Determination of Dimethyl Oxalate and Oxalic Acid by Reversed-Phase High Performance Liquid Chromatography

Shi Lei, Li Weisong, Shi Haochun, Xu Chunjian

State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract

A reversed-phase high performance liquid chromatographic (RP-HPLC) separation method with UV photodiode-array detection has been developed for the simultaneous determination of dimethyl oxalate and oxalic acid. The chromatographic analysis was carried out after their elution with acetonitrile and phosphoric acid solution (pH=2.7) whose volume ratio was 60:40 from C18 stationary phase (4.6 mm×150 mm, 5 μm). The flow rate of the mobile phase was 0.7 mL/min for the whole run. The column temperature was 25 ℃ and the detection wavelength was 254 nm. Effects of pH and other conditions on the separation were investigated. Under the optimized conditions, the liner correlation coefficients of oxalic acid and dimethyl oxalate were 0.9996 and 0.9998, respectively. The relative standard deviations were 0.8%—1.0%. This method was suitable for the determination of dimethyl oxalate and oxalic acid.

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	Shi Lei
	Li Weisong
	Shi Haochun
	Xu Chunjian

Keywords： RP-HPLC； dimethyl oxalate； oxalic acid

Received 2014-04-08;

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Shi Lei, Li Weisong, Shi Haochun, Xu Chunjian.Determination of Dimethyl Oxalate and Oxalic Acid by Reversed-Phase High Performance Liquid Chromatography[J] Chemcial Industry and Engineering, 2016,V33(2): 91-95

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