LmbB2 Catalyzes Bioconversion of Tyrosine to Synthesize L-DOPA
MA Qiang-Qiang, ZHAO Guang-Rong
(Key Laboratory of Systems Bioengineering, Ministry of Education; Department of Pharmaceutical Engineering,School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China)
Abstract L-DOPA is a first-selected drug for Parkinson′s disease in clinical treatment. This article utilizes gene lmbB2 from Streptomyces lincolnensis to construct the engineer Escherichia coli for production of L-DOPA. When 300 mg/L of tyrosine and 3g/L of L-ascorbic acid are added in M9 medium, after fermentation for 32 h at 37℃, the yield of L-DOPA were 285mg/L, and the bioconversion rate was 95%.
MA Qiang-Qiang, ZHAO Guang-Rong.LmbB2 Catalyzes Bioconversion of Tyrosine to Synthesize L-DOPA[J] Chemcial Industry and Engineering, 2014,V31(4): 75-79
2014, Vol. 31 
