大孔树脂-C18柱联用分离纯化<em>β</em>-石竹烯及其抗氧化性研究

摘要利用减压浓缩结合乙醇提取工艺从转基因微藻培养液中获得β-石竹烯粗提液,通过建立大孔树脂联合C18球形柱分离方法对产物进一步浓缩纯化并考察其抗氧化能力。从6种不同类型的大孔树脂中筛选出HPD-100型大孔树脂作为β-石竹烯的最适吸附剂,通过对静态吸附与解吸附过程中温度、pH值、时间和解吸剂的优化,使静态吸附与解吸率分别达到87.8%和80.8%。在动态吸附解吸实验中,初始上样流速采用2 BV·h^-1,上样体积28 BV时,吸附率达98.0%。采用无水乙醇以1.5 mL·min^-1流速洗脱,洗脱体积7 BV,解吸率达64.8%。随后,洗脱液采用C18柱,以甲醇作为流动相,上样体积5 mL,洗脱流速1.0 mL·min^-1,收集到的样品中β-石竹烯质量分数达99.0%,较粗提液提高了63.2%,表明该分离方法效果显著。采用得到的β-石竹烯进行体外体内抗氧化能力测试,发现在1 000 μmol·L^-1时对DPPH、ABTS和·O^-₂清除能力分别为82.2%、76.7%和82.4%,添加10 μmol·L^-1时PC12细胞内ROS含量降低了40.8%。此外,分子对接模拟表明β-石竹烯与SOD、CAT、POD和GPX均具有良好的结合效果。

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	何维刚
	孙承骏
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	陶昱恒
	严生虎
	王利群
	卿青

关键词： β-石竹烯大孔树脂吸附分离纯化抗氧化

Abstract： The crude extract of β-caryophyllene was obtained from the transgenic microalgae culture solution by reduced pressure concentration combined with ethanol extraction process, and the products were further concentrated and purified by establishing a macroporous resin combined with C18 spherical column separation method. Six different types of macroporous resin were investigated, and HPD-100 macroporous resin was selected as the optimal adsorbent for β-caryophyllene. The separation and purification conditions of crude extract were optimized respectively under static and dynamic adsorption-desorption conditions. Through the optimization of temperature, pH, time and desorption agent during static adsorption and desorption, the static adsorption and desorption rates reached 87.8% and 80.8% respectively. In the dynamic adsorption and desorption experiment, the initial loading flow rate was 2 BV·h^-1, the loading volume was 28 BV, the adsorption rate was 98.0%. The desorption rate reached 64.8% using ethanol as eluent at a flow rate of 1.5 mL·min^-1 with the elution volume of 7 BV. Subsequently, 5 mL eluate was loaded to a C18 column with methanol as the mobile phase and the elution flow rate of 1.0 mL·min^-1. The purity of β-caryophyllene in the collected sample reached 99.0% which increased 63.2% compared with the crude extract, indicating the efficiency of this method. In vivo and in vitro antioxidant capacity tests were conducted using the purified β-caryophyllene. The clearance capacity for DPPH, ABTS, and ·O^-₂ was 82.2%, 76.7%, and 82.4%, respectively, at 1 000 μmol·L^-1 and 40.75% reduction in PC12 cells at the addition of 10 μmol·L^-1. Moreover, molecular docking simulations indicated that β-caryophyllene had excellent affinity to SOD, CAT, POD and GPX.

Keywords： β-caryophyllene macroporous resin adsorption separation and purification antioxidant

Received 2023-03-07;

Fund:常州市科技计划(CJ20220217);2022年江苏省青蓝工程项目资助(SCZ2210200010)。

Corresponding Authors: 卿青,副教授,qqing@cczu.edu.cn。 Email: qqing@cczu.edu.cn

About author: 何维刚(1997—),男,硕士研究生,现从事天然产物中萜类化合物的分离纯化工艺方面的研究。

引用本文:

何维刚, 孙承骏, 吕世钧, 陶昱恒, 严生虎, 王利群, 卿青.大孔树脂-C18柱联用分离纯化β-石竹烯及其抗氧化性研究[J]. 化学工业与工程, 2025,42(2): 35-44

HE Weigang, SUN Chengjun, Lü Shijun, TAO Yuheng, YAN Shenghu, WANG Liqun, QING Qing.Study on the process of macroporous resin-C18 column separation and purification of β-caryophyllene and its antioxidation properties[J]. Chemcial Industry and Engineering, 2025,42(2): 35-44

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