Abstract The deacetylation of crab chitin was investigated in this paper.The effects of the main influencing factors such as temperature,concentration of alkali and reaction time on the degree of deacetylation and the value of intrinsic viscosity of chitosan were discussed.The optium conditions are:reaction time 8 hours,temperature 65℃ and concentration of NaOH 47%.Determination of [η] indicated that the degradation of the acid soluble fractions is negligible when deacetylation was performed under a nitrogen purge at 100℃,especially as the reaction is intermittent.