Immobilization of Aminoacylase on DEAE-E/H

化学工业与工程

Chemcial Industry and Engineering

2006, Vol. 23

Issue (3) :206-210 DOI:

Article

Current Issue | Next Issue | Archive | Adv Search

<< | >>

Immobilization of Aminoacylase on DEAE-E/H

GUO Hong-yan,ZHAO Lin,YAN Xiao-min,SONG Zheng-xiao(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China)

(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China

Abstract	Reference	Related Articles

Download: PDF (314KB) HTML () Export: BibTeX or EndNote (RIS) Supporting Info

Abstract Aminoacylase from Aspergillus oryzae was immobilized on DEAE-E/H(functionalized poly glycidylmethacrylate).Effects of amount of DEAE(diethylaminoethyl),surface area and pore diameter on the activity of immobilized aminoacylase were investigated.Some immobilization factors,such as concentration of enzyme suspension,immobilization time and the way of immobilization,were studied on resin Ⅱ.While the concentration of enzyme suspension and immobilization time are 2.0 mg/mL and 8 h,the activity of complex of resion Ⅱ-aminoacylase is 980 U/g.Scanning electron micrographs(SEM) revealed the structures of the resin.

	Service

	Email this article
	Add to my bookshelf
	Add to citation manager
	Email Alert
	RSS
	Articles by authors

Keywords：

Received 2006-05-15; published 2006-05-15

Cite this article:

GUO Hong-yan,ZHAO Lin,YAN Xiao-min,SONG Zheng-xiao.Immobilization of Aminoacylase on DEAE-E/H[J] Chemcial Industry and Engineering, 2006,V23(3): 206-210

[1]	彭珍荣.微生物资源与氨基酸的生产和应用[J].化学与生物工程.2003,20(6):7-8
[2]	张伟国.氨基酸生产技术及其应用[M].北京:中国轻工业出版社,1997.
[3]	CHIBATA I,TETSUYA TOSA,TADASHI SATO.Productionof L-amino acids by aminoacylase absorbed on DEAE-Sphedax[J].Methods in Enzymology.1976,44(51):746-759
[4]	TOOGOOD HELEN S,TAYLOR IAN N,BROWN ROB C,et al.Immobilization of the thermostable L-aminoacylasefrom Thermococcus litoralis to generate a reusable industrialbiocatalyst[J].Biocatalysis and Biotransformation.2002,20(4):241-249
[5]	LEE PAT M,LEE KONG H,SIAW YEW S.Immobilizationof aminoacylase in polyethylenimine stabilized calciumalginate beads forL-phenylalanine production[J].Biomaterials,Artificial Cells,and ImmobilizationBiotechnology,1993,21(4):563-570.
[6]	LI Min-qin,HE Bing-lin.Immobilization of aminoacylase onmodified methyl acrylate-divinylbenzene copolymers[J].Chinese Journal of Reactive Polymers,1993,2(1):34-41.
[7]	LI Min-qin,WANG Dao-bin,ZHOU Ju-yan,et al.Studieson immobilization of aminoacylase from Aspergillus oryzaeon macroporous carriers[J].Science in China,Series B:Chemistry,Life Sciences&Earth Sciences.1993,36(7):778-784
[8]	徐东,曹竹安.国产大孔树脂固定化氨基酰化酶[J].清华大学学报(自然科学版),1990,30(6):33-38.
[9]	何炳林,黄文强.离子交换和吸附树脂[M].上海:上海科技教育出版社,1992.
[10]	孙敏莉,王强斌.DEAE-磁性纳米粒子提纯质粒的研究[J].上海第二医科大学学报,2003,23(6):512-514.
[11]	李民勤,王道宾,周菊岩,等.高分子载体对米曲霉氨基酰化酶的固定化研究[J].离子交换与吸附.1993,9(3):199-203
[12]	WANG Dao-bin,ZHOU Ju-yan,LI Min-qin,et al.Immobilization of aminoacylase from Aspergillus oryzae onfunctionalized acrylamide-methylacrylate copolymers[J].Progress in Natural Science,1994,4(2):167-172.
[13]	韩际宏,姜坪,王道宾,等.氨基酰化酶的固定化及其对DL-蛋氨酸的光学拆分[J].离子交换与吸附,1993,9(3):107-113.