Technology of Solid-Phase Synthesis of Neurotensin

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Abstract Neurotensin(NT)is a kind of bioactive peptide which is composed of 13 amino acids.It has multi-functions,especially in pain relieving and blood hypertension.Thus it has broad applications as potential nerve medicines in the field of medicial area.NT was synthesized successfully from C-terminal to N-terminal according to the amino acids sequence with the raw material of Fmoc-amino acids and Wang resin as the vector.The influences of combination,deblock and cleavage reagents on the final result of synthesis were discussed.The optimized technology of NT synthesis was as follows:using DCM as the coupling solvent,TBTU/HOBT/DIEA as the coupling reagent,resin with TFA/H_2O/thioanisole/Phenol/EDT(Volune ratio of 82∶ 5∶5∶6∶2)as the cleavage condition,the reaction time 3 h.The yield of crude product was 61.8%,the purity of the product was 46%.

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Received 2009-07-15; published 2009-07-15

Cite this article:

WU Lei,REN Yun-xia,JING Wen-peng.Technology of Solid-Phase Synthesis of Neurotensin[J] Chemcial Industry and Engineering, 2009,V26(4): 292-297

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