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Chemcial Industry and Engineering 2010, Vol. 27 Issue (3) :189-194    DOI:
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Isolation and Characterization of a Hydrocarbon-Degrading Bacterial Strain
REN Hua-feng,ZHANG Yu-shan,WANG Jing,ZHANG Xiao-qing,CHENG Yu (The Institute of Seawater Desalination and Multipurpose Utilization,State Oceanic Administration,Tianjin 300192,China)
(The Institute of Seawater Desalination and Multipurpose Utilization,State Oceanic Administration,Tianjin 300192,China

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Abstract A bacterial strain SY095 was isolated from the sludge samples polluted by the municipal solid waste in the beach of Bohai sea. It was identified as Rhodococcus sp. according to its phenotype,physio-logical and chemical properties,and its 16S rRNA gene sequence. The results indicated that strain SY095 could produce bioemulsifier efficiently when n-hexadecane was used as carbon source. The opti- mal conditions for the synthesis of bioemulsifier were: 10 g /L of n-hexadecane,media initial pH 7. 2, cultivation was carried out for 2 d at 30 �� on a rotary shaker at 160 r /min. Under these conditions the surface tension of culture decreased to the lowest value,around 32 mN /m,after 2 d. The critical micelle concentration (CMC) of the bioemulsifier was 75 mg /L.
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Received 2010-05-15; published 2010-05-15
Cite this article:   
REN Hua-feng,ZHANG Yu-shan,WANG Jing,ZHANG Xiao-qing,CHENG Yu .Isolation and Characterization of a Hydrocarbon-Degrading Bacterial Strain[J]  Chemcial Industry and Engineering, 2010,V27(3): 189-194
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