The Exploration of Yeast Chassis for 7-Dehydrocholesterol Biosynthesis

化学工业与工程

Chemcial Industry and Engineering

2017, Vol. 34

Issue (4) :83-90 DOI: 10.13353/j.issn.1004.9533.20151094

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The Exploration of Yeast Chassis for 7-Dehydrocholesterol Biosynthesis

Su Wan, Liu Yue, Zhou Xiao, Wang Ying

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract

7-Dehydrocholesterol is an important intermediate in synthesizing vitamin D₃, while the chemical synthesis and the biological transformation techniques could not satisfy the requirement of industrial production so far. The emerge of synthetic biology has provided another choice for the heterologous synthesis of secondary metabolites. Saccharomyces cerevisiae and Yarrowia lipolytica are model organisms in industry. The ergosterol biosynthesis may suppress the 7-dehydrocholesterol production in S.cerevisiae. The combined strategy of knocking out erg5, a non-essential gene for ergosterol synthesis, and introducing human dhcr24 gene encoding C24 reductase resulted in 2.62 mg·L^-1 7-dehydrocholesterol production in shake flask culture. The ergosterol did not show any influence on the synthesis of 7-dehydrosterol in Y.lipolytica. After integrating the human dhcr24 gene into rDNA site of the genome, the highest production of 7-dehydrosterol in Y.lipolytica was 27.91 mg·L^-1. The results in this study provide a reference of the discovery of suitable chassis for the biosynthesis of sterol metabolites.

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	Su Wan
	Liu Yue
	Zhou Xiao
	Wang Ying

Keywords： 7-dehydrocholesterol； synthetic biology； chassis； yeast

Received 2015-05-19;

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Su Wan, Liu Yue, Zhou Xiao, Wang Ying.The Exploration of Yeast Chassis for 7-Dehydrocholesterol Biosynthesis[J] Chemcial Industry and Engineering, 2017,V34(4): 83-90

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