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Chemcial Industry and Engineering 2015, Vol. 32 Issue (6) :74-79    DOI: 10.13353/j.issn.1004.9533.20131097
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Effects on Xylose Fermentation in Recombinant Saccharomyces cerevisiae with the Heterologous Transhydrogenase NNT
Yang Ke, Zhang Guochang, Chen Xun
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract 

The recombinant Saccharomyces cerevisiae which encoded the genes xylose reductase (XR) and xylitol dehydrogenase(XDH) from Pichia stipitis, can utilize xylose. However, XR and XDH prefer NADPH and NAD+ respectively, create cofactor imbalance. As a result, xylose fermentation to ethanol with recombinant Saccharomyces cerevisiae was ineffective. NADPH and NADH can be converted to one another by transhydrogenase. So in this study, the gene NNT from Aspergillus niger was introduced into recombinant S.cerevisiae. The result of experiment demonstrated the expression protein of gene NNT was positioned at chondriosome. Gene NNT was expressed by the promoters pPGK1, pCCW12 and pHXT7 respectively. Under micro aerobic condition of xylose fermentation, the formation of glycerol was decreased and yield of ethanol was increased in the yeast carrying NNT. In recombinant yeasts with promoter pPGK1 and pCCW12, the yield of xylitol decreased by 86.3% and 49.3%, and the yields of ethanol increased by 16.7% and 12.7% respectively, which illustrated the transhydrogenase improved cofactor imbalance of xylose fermentation and increased ethanol conversion.

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Yang Ke
Zhang Guochang
Chen Xun
KeywordsSaccharomyces cerevisiae   transhydrogenase;   xylose;   glycerol;   ethanol     
Received 2013-04-01;
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Yang Ke, Zhang Guochang, Chen Xun.Effects on Xylose Fermentation in Recombinant Saccharomyces cerevisiae with the Heterologous Transhydrogenase NNT[J]  Chemcial Industry and Engineering, 2015,V32(6): 74-79
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