Effect of Overexpression of Succinate Exporter SucE on Anaerobic Succinate Production by <em>Corynebacterium glutamicum</em>

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Abstract Corynebacterium glutamicum ATCC 13032 produces succinate through the reductive tricarboxylic acid cycle under anaerobic conditions. Efficient export of succinate into the culture supernatant is important to increase its production. In this study, sucE gene overexpression was introduced into strain C. glutamicum XZ. The resulting strain C. glutamicum XZ(pExhsucE)showed a 7% increase in succinate yield and a 19% increase in succinate productivity. Also, the relative flux of the glyoxylate pathway increased by 50%. Using two-stage culture, succinate titer up to 518 mmol·L^-1 was achieved with a specific productivity of 0.95 mmol gCDW^-1·h^-1and a yield of 1.5 mol (mol glucose)^-1.

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Keywords�� succinate exporter�� metabolic flux analysis�� two-stage fermentation�� Corynebacterium glutamicum

Received 2013-05-08;

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Xia Huihua, Yin Guomin, Zhu Nianqing, Chen Tao.Effect of Overexpression of Succinate Exporter SucE on Anaerobic Succinate Production by Corynebacterium glutamicum[J] Chemcial Industry and Engineering, 2015,V32(2): 74-79

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