| Abstract The Rhodococcus sp. H-412 screened in our lab, which could desulfurize via 4S pathway, was selected as biocatalyst to study the desulfurization performance by its growing cells, dibenzothiophene (DBT) and n-hexadecane were used for model organic-sulfur compound and model oil, respectively. The desulfurization efficiency (XDBS) of growing cells is higher than that of resting cells in the same biomass concentration when the oil phase was 20% (v/v). When initial biomass concentration was 0.048 g/L and DBT concentration was 0.25 mmol/L, the higher XDBS was reached 57% under 20% (v/v) of hexadecane. The desulfurization by growing cells was carried out under the condition of 0%, 5%, 10%, 20% (v/v) of hexadecane, DBT consumption and 2-HBP formation increased with the increasing of oil phase, and the highest XDBS was reached when the hexadecane was 20%. Biomass growth reduced with the increasing of oil volume, under oil-free condition, the biomass reached highest value. |
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2012, Vol. 29 
