Abstract Direct heating explosion bagasse and the cellulase (Onozuk RS)bought from Yakult Co. Ltd.(Japan)were used in enzymatic hydrolysis and membrane separation experiments. The membrane materials and molecular weight cutoff were selected ;the separation characteristics were determined ,and the washing methods for membrane fouling were also tried. The experimental results indicated that polyether sulfone membrane of MW cutoff of 10 000 had 100% interception rate for the cellulose; the adsorption of the hydrolyszate had no appreciable effect on the flux through the membrane ; the enzyme recovery rate was only 56% through membrane separation in the experimental conditions; the PH of the hydrolyzate had no effect on the flux through the membrane i when the velocity of liquid wae smallen than 2 m/min, the velocity of liquid through the surface of membrane had no effect on the flux through the membrane; when the velocity was 2-4 m/min, the flux increased with the velocity of liquid; and when the velocity was more than 4 m/min,the velocity had no effect on the flux; the membrane being washed with 1% P3-ultrasil 56(bought from Henkel, Japan)can recover 90% of its flux in a washing time of half an hour. |
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