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ժҪ ��Ի�ѧ��ݶ��Ϊ��ɢ�ƶ��MaxwellStephan��ɢ��(MS��),��ƽ��ɢģ��,��QSepharoseFF��ţѪ��׵��(BSA)�Ķ�̬��ݽ��ģ��,��ϵõ��BSA�ı��ɢ�Ϳ��ɢϵ��о��ɢϵ��pH��͡��Ũ��Ӷ��;��ɢϵ��pH��ӡ��Ũ�Ƚ��Ͷ��,�浰��ʳ�ʼŨ��Ӷ��С��ڱ��ɢ��ݺ��Ϊ��²��Fick��ʱ�õ��ı��ɢϵ��ǵ��Ũ�ȵĺ��,�Ҵ��ڸ��MS��̵õ��ı��ɢϵ��

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Abstract�� Parallel diffusion model was combined with the chemical potential gradient driving diffusion equation (Maxwell-Stephan Equation,or MS Equation in shortened form) to fit the uptake curves of BSA in anion ion-exchange adsorbent Q-Sepharose FF to study the influence of pH,salt concentrations and initial protein concentrations on diffusion coefficients in ion-exchange adsorbent.It shows that surface diffusion coefficients are larger with lower pH and higher salt concentration. Pore diffusion coefficients decrease with higher initial concentration of protein probably due to the blocking effect of adsorbed protein molecules to pores and increase with increasing pH and decreasing salt concentration. It is pointed out that in the case of surface diffusion not being negligible and non-linear adsorption,diffusion coefficients become a function of concentration when Fick law is adopted and it is necessary to adopt MS equation to describe the diffusion flux in the adsorbent.

Keywords��

Received 2005-09-15; published 2005-09-15

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��ѩ��,ʷ��,��,��.��ӽ��е��ɢϵ��[J]. ��ѧ��ҵ�빤��, 2005,22(5): 362-366

SU Xue-li1, SHI Qing-hong1, WU Yu-long2, ZHANG Meng1.Diffusion Coefficients of Protein in Ion-Exchange Adsorbent[J]. Chemcial Industry and Engineering, 2005,22(5): 362-366

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