Abstract The technological condition for solubilization of the recombinant human Interleuk in\|6(rhIL 6) inclusion body produced from E.coli was studied. The optimal solubilization condition was obtained:the inclusion body was solubilized at 4℃ in 50mmol/L Tris\|HCL(pH=8.4),8mol/L urea and 50 mM β ME at a concentration of 10mg/mL for about 1h. Then nearly all the target protein was solubilized and the solubilized protein concentration was solubilized and the solubilized protein concentration was about 1.3 mg/mL and the purity was up to 50%.
WU Lei,ZHANG Ying, GAN Yi\|ru,SUN Jiang\|li, ZHANG Hao sen .A Study for Solubilization of rhIL 6 Inclusion Body Produced from E.Coli[J] Chemcial Industry and Engineering, 2000,V17(6): 321-325
2000, Vol. 17 
