Study on Culture Technological Conditions of Klebsiella Pneumoniae
FANG Wen 1,WU Lei 1,GE Xin 2,ZHANG Ying 1,GAN Yi-ru 1 (1. School of Chemical Engineering, Tianjin University, Tianjin 300072,China; 2.Tianjin Medical University, Tianjin 300070,China)
(1. School of Chemical Engineering, Tianjin University, Tianjin 300072,China; 2.Tianjin Medical University, Tianjin 300070,China
Abstract The cultivation of Klebsiella pneumoniae in flask was studied. The medium recipe suitable to Kp-9 strain growth and expression of the capsular glycoprotein was proposed. The ingredients of the recipe include glucose 1%, soya peptone 1.5%, beef extract 0.2%, yeast extract 0.2%,tryptone 0.5%,NaCl 0.5, K 2HPO 4 0.35% and KH 2PO 4 0.15%. The suitable culture conditions are 160r/min of rotate speed, 20% of filling capacity and 2% of inoculum volume.
FANG Wen 1,WU Lei 1,GE Xin 2,ZHANG Ying 1,GAN Yi-ru 1 .Study on Culture Technological Conditions of Klebsiella Pneumoniae[J] Chemcial Industry and Engineering, 2002,V19(1): 6-11
2002, Vol. 19 
