Influence of Inhibitors in Lignocellulosic Hydrolysates on Ethanol Fermentation of Cellobiose-Utilizing <em>Saccharomyces cerevisiae</em> Strain

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Abstract

During the pretreatment of lignocellulosic materials for microbial fermentation, a wide range of inhibitors were generated, including furans, phenols and weak acids etc. These inhibitors would inhibit the cell growth and sugar utilization of fermentation microorganisms. In this study, three typical inhibitors, furfural, phenol and acetic acid, were used to investigate their influence on the genetically engineered Saccharomyces cerevisiae SyBE001603. The tolerance of strain to the three inhibitors when using cellobiose or glucose as the sole carbon source was evaluated. It indicated that furfural could inhibit both the cellobiose and glucose utilization capability of the strain, and more severe inhibition occured with higher furfural concentration. At a phenol concentration less than or equal to 0.5 g/L, the cellobiose utilization was facilitated, while at the phenol concentration of 1.5 g/L, the cell growth and cellobiose utilization were strongly inhibited. Acetic acid exhibited significant inhibition on the cell growth and the cellobiose utilization, and the cellobiose consumption rate and ethanol productivity were strongly reduced as the acetic acid concentration increased. However, when using glucose as the sole carbon source, acetic acid (0.5~4.0 g/L) would accelerate the glucose utilization. In addition, it was observed that the synergistic inhibition effects of multiple inhibitors were more severe than that of single inhibitor.

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	Articles by authors
	Hu Menglong
	He Linwei
	Li Bingzhi
	Yuan Yingjin

Keywords： cellulosic ethanol； cellobiose； inhibitor； lignocellulosic hydrolysate； synthetic biology

Received 2014-05-23;

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Hu Menglong, He Linwei, Li Bingzhi, Yuan Yingjin.Influence of Inhibitors in Lignocellulosic Hydrolysates on Ethanol Fermentation of Cellobiose-Utilizing Saccharomyces cerevisiae Strain[J] Chemcial Industry and Engineering, 2016,V33(2): 77-84

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