Advances in Single Cell Sequencing

Abstract High through-put sequencing technologies have revolutionized the field of genomics, enabling fast and cost-effective generation of genome-scale sequence data with exquisite resolution and accuracy. Genome sequencing currently requires DNA from pools of numerous nearly identical cells, and the data obtained to date are averages of ensembles of cells. However, it is not certain whether the same features are the same inside an individual cell. Accordingly, important information may be masked by the averaging process. Single cell sequencing is a kind of high through-put sequencing technologies on the scale of individual cells. Compared with conventional through-put sequencing, it could be a powerful approach for studying uncultivated microorganism and the genetic heterogeneity of individual cells with the same phenotype. Single cell sequencing technology includes single cell isolation, cell lysis and DNA purification, whole genome amplification, genome sequencing and data analysis. Here, we review single-cell genome sequencing, analyze the advantages and disadvantages of current technologies and describe the recently recommended improvements.

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Keywords： single cell sequencing; single cell isolation; whole genome amplification (WGA); multiple displacement amplification (MDA)

Received 2014-03-03;

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Dong Yan, Song Chengcheng, Huang He.Advances in Single Cell Sequencing[J] Chemcial Industry and Engineering, 2015,V32(1): 71-78

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