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Chemcial Industry and Engineering 2015, Vol. 32 Issue (1) :64-70,78    DOI: 10.13353/j.issn.1004.9533.2015.01.013
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Xylose Fermentation Performance of Saccharomyces cerevisiae with Different Combination of the Overexpressed Non-Oxidative Pentose Phosphate Pathway Genes
Sun Jinyun, Gao Wenxuan, Ding Wentao, Liu Wen, Chen Xun
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract To study the influence of non-oxidative pentose phosphate pathway (PPP) on xylose utilization in Saccharomyces cerevisiae, different combinations of PPP genes TKL1, RPE1 and RKI1 were overexpressed in the TAL1-enhanced yeast strain, which also had the double-copy integration of upstream pathway genes XYL1, XYL2 and XKS1. Performance of recombinant yeasts was investigated at both 5% and 8% xylose as well as glucose and xylose mixture, results showed that overproduction of PPP genes stimulated cell growth and xylose conversion at both xylose concentrations, concomitant with higher ethanol yield at the expense of xylitol and glycerol formation, and all recombinant strains can use up 5% xylose within 36 to 48 h. Yeast strains of S. cerevisiae with four PPP genes co-overexpressed showed more advantages, and under the 8% xylose concentration, ethanol yield was about 0.337 g/g xylose, 7.86% higher than parent strain. It indicated that co-overproduction of all PPP genes may be the top priority for enhancing xylose metabolism in S. cerevisiae strains.
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KeywordsSaccharomyces cerevisiae;   non-oxidative pentose phosphate pathway;   xylose;   ethanol     
Received 2013-04-02;
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Sun Jinyun, Gao Wenxuan, Ding Wentao, Liu Wen, Chen Xun.Xylose Fermentation Performance of Saccharomyces cerevisiae with Different Combination of the Overexpressed Non-Oxidative Pentose Phosphate Pathway Genes[J]  Chemcial Industry and Engineering, 2015,V32(1): 64-70,78
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