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��ѧ��ҵ�빤�� 2003, Vol. 20 Issue (6) :447-451    DOI:
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Preparation and Some Properties of Egg Yolk ImmunoglobulinY(IgY) Against Influenza Virus
CHEN Dai-mei, SUN Shu-qing(School of Science,Tianjin University, Tianjin 300072, China)
(School of Science,Tianjin University, Tianjin 300072, China
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Abstract�� Influenza virus was used as antigen to immunize laying chicken.The immunized egg yolks were isolate by two-step salt outing and one-step gel filtration methods to obtain purified IgY,of which purity is over 95% by SDS-PAGE analysis.The purified IgY was digested with pepsin (pH=4,37��) to obtain smaller antibody Fab.The reaction activities of IgY and Fab was analyzed comparatively by competitive ELISA method,which shows digested IgY remaining about 70% of initial activity.
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Received 2003-11-15; published 2003-11-15
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�´�÷,������.�����в������ѻ������򵰰�IgY���Ʊ�������[J].  ��ѧ��ҵ�빤��, 2003,20(6): 447-451
CHEN Dai-mei, SUN Shu-qing.Preparation and Some Properties of Egg Yolk ImmunoglobulinY(IgY) Against Influenza Virus[J].  Chemcial Industry and Engineering, 2003,20(6): 447-451
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