Mn<sub>3</sub>O<sub>4</sub>纳米颗粒的类漆酶活性及其对吖啶橙的降解性能研究

摘要为了构建具有漆酶活性的无机纳米酶以克服天然漆酶的提取纯化过程复杂、稳定性较差和成本较高的缺点,采用化学沉淀法制备了Mn₃O₄纳米颗粒,以2,4-二氯苯酚为底物对其类漆酶活性进行了评估,并探究了该纳米颗粒对水中吖啶橙的降解能力。结果显示Mn₃O₄纳米颗粒具有类似漆酶的氧化还原活性,酶动力学实验结果显示在相同的质量浓度下,Mn₃O₄纳米酶与天然漆酶相比具有更高的催化效率;此外,Mn₃O₄纳米酶具有更宽的温度耐受范围(25~75℃),室温储存稳定性显著高于天然漆酶。Mn₃O₄纳米颗粒在氧化还原介质HBT存在下可以快速高效去除水溶液中的有色染料吖啶橙,5 min内的去除效率可达70%。研究结果表明,Mn₃O₄作为漆酶类纳米酶,催化活性较高,储存稳定,制备过程简单,成本低廉,在染料废水处理领域具有良好的应用前景。

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	韩倩
	葛志强

关键词： Mn₃O₄纳米颗粒漆酶纳米酶吖啶橙降解

Abstract： Natural laccase suffers from several inherent drawbacks, such as complex purification process, poor stability and high cost, significantly constraining their practical applications. The construction of inorganic nanozymes with laccase activity is a promising strategy to overcome these limitations. In this study, Mn₃O₄ nanoparticles were prepared using a chemical precipitation method. The laccase-like activity was evaluated using 2,4-dichlorophenol as the substrate. Additionally, the removal performance of the nanoparticles towards AO in aqueous solution was investigated. This study shows that Mn₃O₄ nanoparticles have redox activity similar to laccase, and enzyme kinetics experiments demonstrate that Mn₃O₄ nanozyme has higher catalytic efficiency than natural laccase at the same concentration. Furthermore, Mn₃O₄ nanozyme have a wider temperature (25—75 ℃) and pH (6—7) tolerance range, and the storage stability at room temperature is significantly higher than that of natural laccase. In the presence of the redox mediator HBT, Mn₃O₄ nanoparticles can swiftly and effectively remove AO from aqueous solution, with a removal efficiency of 70% in just 5 min. Therefore, Mn₃O₄, as a laccases-like nanozyme, has excellent developing potential in treatment of dye wastewater due to high catalytic activity, superior storage stability, simplicity of preparation and low cost.

Keywords： Mn₃O₄ nanoparticles laccase nanozyme acridine orange degradation

Received 2022-09-01;

Corresponding Authors: 葛志强,副教授,gezhiq@tju.edu.cn Email: gezhiq@tju.edu.cn

About author: 韩倩(1998—),女,硕士研究生,现从事无机纳米酶方面的研究。

引用本文:

韩倩, 葛志强.Mn₃O₄纳米颗粒的类漆酶活性及其对吖啶橙的降解性能研究[J]. 化学工业与工程, 2025,42(1): 59-68

HAN Qian, GE Zhiqiang.Laccase-like activity of Mn₃O₄ nanoparticles and its application in degrading acridine orange[J]. Chemcial Industry and Engineering, 2025,42(1): 59-68

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