海洋石油降解菌固定化小球的制备及其对含油海水的降解特性

摘要以实验室前期筛选分离得到的海洋石油降解菌SI-JHS为供试菌，在不同包埋条件下对SI-JHS进行固定化以提高其对含油海水的降解率，考察了pH值、盐度和温度等海水环境条件对固定化小球降解特性的影响。实验结果表明，包埋剂选用10%（质量分数，下同）的聚乙烯醇（PVA）和1%的海藻酸钠（SA）混合液，交联剂选用含3% CaCl₂的硼酸饱和溶液，包菌量为20%，活性炭添加量为5%时制备出的固定化小球成球性好，具有较好的传质性能和机械强度。SI-JHS固定化小球对含油海水的最适宜降解条件为：pH值7.0~7.5、盐度3%~4%、温度30~35℃。SI-JHS固定化小球对含油海水的降解率为97.8%，较游离菌提高了22.6%，固定化小球对海水中石油的降解过程符合准一级降解动力学模型。

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	姜天翔
	张爱君
	王文华
	王静
	任华峰

关键词：海洋石油降解菌固定化小球含油海水降解效果

Abstract： In this work, a marine petroleum-degrading bacteria (SI-JHS) which was insolated previously in our laboratory, was immobilized at different embedding conditions to increase the degradation efficiency for petroleum-containing seawater. The effects of environmental conditions in seawater such as pH, salinity and temperature on degradation characteristics of immobilized beads were investigated. Immobilized beads prepared by 10% polyvinyl alcohol (PVA) and 1% sodium alginate (SA) as embedding material, and saturated boric acid solutions containing 2% calcium chloride (CaCl₂) as crosslinker. The better mass transfer performance and greater mechanical strength of immobilized beads were obtained when bacteria entrapment reached 20% and 5% activated carbon was added. The best degradation conditions for immobilized beads to degrade petroleum-containing seawater were:pH at 7.0~7.5, salinity at 3%~4% and temperature at 30~35℃. The degradation rate of petroleum by SI-JHS-immobilized beads was 97.8%, which was 22.6% higher than free bacteria. The degradation process could be followed by the pseudo first order dynamic model.

Keywords： marine petroleum-degrading bacteria； immobilized beads； petroleum-containing seawater； degradation efficiency

Received 2017-09-28;

Fund:中央级公益性科研院所基本科研业务费专项资金项目（K-JBYWF-2016-G11，K-JBYWF-2016-T9），国家重点研发计划"海洋环境安全保障"重点专项（2016YFC1402307）。

Corresponding Authors: 王静,E-mail:wang_nana@163.com。 Email: wang_nana@163.com

About author: 姜天翔(1979-),男,工程师,主要从事海水净化与水再利用技术研究。

引用本文:

姜天翔, 张爱君, 王文华, 王静, 任华峰.海洋石油降解菌固定化小球的制备及其对含油海水的降解特性[J]. 化学工业与工程, 2019,36(3): 35-41

Jiang Tianxiang, Zhang Aijun, Wang Wenhua, Wang Jing, Ren Huafeng.Preparation of Immobilized Marine Petroleum-Degrading Bacteria and its Degradation Characteristics or Petroleum-Containing Seawater[J]. Chemcial Industry and Engineering, 2019,36(3): 35-41

[1] Wang X, Wang X, Liu M, et al. Adsorption-Synergic biodegradation of diesel oil in synthetic seawater by acclimated strains immobilized on multifunctional materials[J]. Marine Pollution Bulletin, 2015, 92(1/2):195-200
[2] 李艳梅, 曾文炉, 余强, 等. 海洋溢油污染的生态与健康危害[J]. 生态毒理学报, 2011, 6(4):345-351 Li Yanmei, Zeng Wenlu, Yu Qiang, et al. Adverse effect of marine oil spills on human health and ecosystem:A review[J]. Asian Journal of Ecotoxicology, 2011, 6(4):345-351(in Chinese)
[3] Pontes J, Mucha A P, Santos H, et al. Potential of bioremediation for buried oil removal in beaches after an oil spill[J]. Marine Pollution Bulletin, 2013, 76(1/2):258-265
[4] Radwan S, Al-Hasan R, Salamah S, et al. Bioremediation of oily sea water by bacteria immobilized in biofilms coating macroalgae[J]. International Biodeterioration & Biodegradation, 2002, 50(1):55-59
[5] 李欣, 凌婉婷, 刘静娴, 等. 固定化菌剂对污水和牛粪中雌二醇和已烯雌酚的去除作用[J]. 环境科学, 2015, 36(7):2581-2590 Li Xin, Ling Wanting, Liu Jingxian, et al. Immobilization of estrogen-degrading bacteria to remove the 17β-estradiol and diethylstilbestrol from polluted water and cow dung[J]. Environmental Science, 2015, 36(7):2581-2590(in Chinese)
[6] Alessandrello M J, Juárez Tomás M S, Raimondo E E, et al. Petroleum oil removal by immobilized bacterial cells on polyurethane foam under different temperature conditions[J]. Marine Pollution Bulletin, 2017, 122(1/2):156-160
[7] 邵基伦, 曹刚, 李紫惠, 等. 包埋固定化异养硝化菌强化处理氨氮有机废水[J]. 中国给水排水, 2015, 31(1):5-9 Shao Jilun, Cao Gang, Li Zihui, et al. Enhanced treatment of organic wastewater containing ammonia nitrogen by embedded immobilized heterotrophic nitrobacteria[J]. China Water & Wastewater, 2015, 31(1):5-9(in Chinese)
[8] Shen Y, Gao J, Li L. Municipal wastewater treatment via co-immobilized microalgal-bacterial symbiosis:Microorganism growth and nutrients removal[J]. Bioresource Technology, 2017, 243:905-913
[9] 任华峰, 张雨山, 王静, 等. 石油烃降解菌的分离鉴定及其产生乳化剂条件[J]. 化学工业与工程, 2010, 27(3):189-194 Ren Huafeng, Zhang Yushan, Wang Jing, et al. Isolation and characterization of a hydrocarbon-degrading bacteria strain[J]. Chemical Industry and Engineering, 2010, 27(3):189-194(in Chinese)
[10] Wu K, Wisecarver K D. Cell immobilization using PVA crosslinked with boric acid[J]. Biotechnology Bioengineering, 1992, 39:447-449
[11] 张爱君, 郝建安, 杨波, 等. 海洋石油降解菌的筛选、鉴定及降解活性[J]. 化学工业与工程, 2015, 32(1):31-36 Zhang Aijun, Hao Jianan, Yang Bo, et al. Isolation and identification of petroleum degrading marine bacteria and its activity[J]. Chemical Industry and Engineering, 2015, 32(1):31-36(in Chinese)
[12] 孙瑞珠, 马玉龙, 张娟, 等. 泰勒菌素降解菌的筛选及其降解动力学研究[J]. 中国环境科学, 2013, 33(4):722-727 Sun Ruizhu, Ma Yulong, Zhang Juan, et al. Isolation and degradation dynamic of a tylosin-degrading strains[J]. China Environmental Science, 2013, 33(4):722-727(in Chinese)