(1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China; 3. Tianjin Institute of Industrial Biotechnology, Tianjin 300308, China; 4. EdinburghTianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin 300072, China)
Abstract：
The external metabolites that can be transferred to/from the cell can greatly affect the analysis results. In this paper, the genome-scale metabolic networks of E. coli and B. subtilis as examples were used to simulate the metabolic states at various conditions where different transport reactions for external metabolites were knocked out. The result showed the preference of different metabolic byproducts were related with their energy states, thus providing some guidance on engineering a strain to improve the production of different metabolic products.
2013, Vol. 30 
