Laser-Induced Mutation of Biodesulfurization Strain Gordonia sp. WQ-01 and Cloning Expression of Monooxygenase Gene (DszC) in Escherichia coli
WAN Tao, HUANG Di, WEN Jian-Ping, WANG Hui-Fang, LIU Lin, ZHANG Shuang
（School of Chemical Engineering and Technology，Tianjin University，Tianjin 300072，China; Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin 300072, China）
Abstract：
Laser-induced mutation was used to get a mutant of biodesulfurization strain Gordonia sp. WQ-01，and the various irradiation time and output power was investigated. Based on the DszC enzyme activity, nucleotide sequence alignment, structure analysis and heterologous expression in Escherichia coli, the relevant desulfurization characteristic was revealed. The results showed that a highefficient desulfurization strain with the efficiency of 0.15 mmol·L-1·h-1 and a reduced desulfurization time (36 h) was achieved under the optimal irradiation condition, which was 15 min and 20.0 mW. Compared with the parent strain, the key enzyme DszC was improved by 33.3%. The nucleotide and amino acid sequence, second and third structure were changed for dszC in the mutant strain, thus resulted in an increase of enzyme activity. The E. coli BL21(DE3) expressing the dszC produced a protein with 45 kDa with IPTG induction.
WAN Tao, HUANG Di, WEN Jian-Ping, WANG Hui-Fang, LIU Lin, ZHANG Shuang.Laser-Induced Mutation of Biodesulfurization Strain Gordonia sp. WQ-01 and Cloning Expression of Monooxygenase Gene (DszC) in Escherichia coli[J]. Chemcial Industry and Engineering, 2013,30(6): 67-73
2013, Vol. 30 
