海洋石油降解菌的筛选、鉴定及降解活性

摘要从渤海入海口的滩涂采集泥沙,采用石油为唯一碳源的筛选培养基,梯度稀释法与划线平板法作为筛选方法,分离、筛选出1株可降解石油的菌株SI-JHS。经过16S rDNA测序及Biolog 95种碳源分析,鉴定该菌株为假交替单胞菌(Pseudoalteromon sp.)。通过改变pH值、温度、盐度和石油含量等实验对SI-JHS进行石油降解试验。研究表明,菌株SI-JHS的最适宜降解条件为:盐度3.5%,pH值7.0,温度35 ℃,在该条件下,SI-JHS对石油降解率可达75.71%。

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关键词：石油降解菌; 分离鉴定; 影响因素

Abstract： A novel petroleum-degrading microbial strain, designated SI-JHS, was isolated from tidal flat sediment in estuary of Bohai, by using serial dilution method and plate streak method in the medium containing petroleum as a sole carbon source. On the basis of 16S rRNA gene sequence analysis and Biolog analysis of 95 carbon sources, the strain was primarily identified as Pseudoalteromon.sp. Effects of pH values, temperature, salinity, concentrations of petroleum on degradation of activities SI-JHS were investigated. The results showed that under the optimal growth conditions, with salinity of 3.5%, pH of 7.0, temperature of 35 ℃, the petroleum could be degraded in the high efficiency of 75.71%.

Keywords： petroleum degradation bacterium; isolation and identification; influencing factors

Received 2013-11-29;

Fund:

海洋公益性行业科研专项经费项目(201305022-5);天津市科技兴海项目(KJXH2011-01);中央级公益性科研院所基本科研业务费专项基金项目(K-JBYWF-2011-G08)。

Corresponding Authors: 张雨山,电话:(022)87890659,E-mail:yushanzhang@hotmail.com。 Email: yushanzhang@hotmail.com

About author: 张爱君(1984-),女,助理工程师,研究方向为海水水质及微生物学。

引用本文:

张爱君, 郝建安, 杨波, 张晓青, 姜天翔, 杜瑾, 张雨山.海洋石油降解菌的筛选、鉴定及降解活性[J]. 化学工业与工程, 2015,32(1): 31-36

Zhang Aijun, Hao Jianan, Yang Bo, Zhang Xiaoqing, Jiang Tianxiang, Du Jin, Zhang Yushan.Isolation and Identification of Petroleum Degrading Marine Bacteria and Its Activity[J]. Chemcial Industry and Engineering, 2015,32(1): 31-36

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