Abstract：
Flocculants were commonly used agents in seawater purification. Seawater flocculants were hot spot research area. In this study, we collected samples from Bohai Gulf. Plate dilution method was applied to isolate bacteria. Improved detection method was developed to screen flocculating active strains. A bacterial strain with high flocculating activity was found. The strain was identified as Bacillus licheniformis by 16S rDNA test and biolog test. It was nominated as Bacillus licheniformis dhs-36 ultimately. According to the flocculating rate analysis of culture, supernatant and broken cell product, we found the flocculating activity of the strain was only in fermentation supernatant. Flocculating active curve showed the strain had best flocculating activity after two days. Following the stability test of flocculating activity, we found the flocculating activity of the strain was sensitive to pH. The strain could only maintain flocculating activity while pH varied from 6 to 10. But it could maintain flocculating activity while temperature varied in a wide range. The flocculating active substance was identified as protein by saccharide, protein, lipid, and nucleic acids qualitative test. High flocculating activity, convenient collecting method of flocculating active substance made this bacteria a promising material in seawater treatment.
HAO Jian-An, YANG Bo, JIANG Tian-Xiang, ZHANG Xiu-Zhi, ZHANG Xiao-Qing, ZHANG Ai-Jun, WANG Jing, ZHANG Yu-Shan.Screening of a Flocculant-Producing Bacterial Strain and Study on Its Flocculating Activity[J]. Chemcial Industry and Engineering, 2013,30(5): 21-25
2013, Vol. 30 
