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化学工业与工程 2013, Vol. 30 Issue (5) :16-20    DOI:
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玉米芯HNO3/HCl预处理及同步糖化发酵制乙醇
 刘晓娟1, 吴晓斌1, 郑文耀2, 张毅民1
(1绿色合成与转化教育部实验室,天津大学化工学院,天津 300072; 2中国乐凯胶片集团公司,河北 保定 071054)
Pretreatment of Corn Cob with HNO3/HCl and Ethanol Production by Simultaneous Saccrification and Fermentation
LIU  Xiao-Juan1, WU  Xiao-Bin1, ZHENG  Wen-Yao2, ZHANG  Yi-Min1
(1Key laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology,
Tianjin University, Tianjin 300072, China; 2China Luckyfilm Group Co., Baoding 071054, China)

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摘要 采用正交实验对玉米芯在2% HNO3/HCl中的水解条件进行优化,得出最适宜的预处理条件为:反应温度120 ℃,反应时间30 min,固含量15%。将经过预处理的玉米芯作为同步糖化发酵的底物,采用单因素实验考查影响发酵的因素,结果表明:在底物浓度为150 g/L、37 ℃、pH值为5.0、纤维素酶用量为30 FPU/g底物、酵母接种量10%、发酵周期72 h时,乙醇的产率可达到76.8%,此时乙醇溶液的浓度为41.4 g/L。
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刘晓娟1
吴晓斌1
郑文耀2
张毅民1
关键词正交实验   玉米芯   HNO3/HCl   同步糖化发酵   乙醇     
Abstract: HNO3/HCl was used to hydrolyze corn cob for sugar releasing. The optimizum conditions were obtained by using orthogonal experiment, with the reaction temperature of 120 ℃, reaction time of 30 min and solid loading of 15%. Taking the pretreated corn cob as substrate, ethanol was produced by simultaneous saccrification and fermentation and the effects on fermentation were investigated. The results showed that the suitable fermentation conditions were reaction temperature of 37 ℃,pH 5.0, enzyme loading of 30 FPU/g substrate, yeast concentration of 10% (w/v), fermentation time of 72 h and with the solid loading of 150 g/L, the final concentration of ethanol was 41.4 g/L, which was 76.8% of the theoretical yield.
Keywords:   
Received 2012-06-09;
Fund:

国家青年基金(21106097)。

Corresponding Authors: 张毅民,E-mail: zhangym@tju.edu.cn。     Email: zhangym@tju.edu.cn
About author: 刘晓娟(1984-),女,硕士研究生。
引用本文:   
刘晓娟1, 吴晓斌1, 郑文耀2, 张毅民1.玉米芯HNO3/HCl预处理及同步糖化发酵制乙醇[J].  化学工业与工程, 2013,30(5): 16-20
LIU Xiao-Juan-1, WU Xiao-Bin-1, ZHENG Wen-Yao-2, ZHANG Yi-Min-1.Pretreatment of Corn Cob with HNO3/HCl and Ethanol Production by Simultaneous Saccrification and Fermentation[J].  Chemcial Industry and Engineering, 2013,30(5): 16-20
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