Morphological Analysis Methods and Main Factors Influencing Morphology of Fliamentous Microorganisms

Related Articles

Abstract Several morphological analysis methods of filamentous microorganisms such as direct observation,digitizing table and image analysis were introduced in this paper.Image analysis reduces subjectivity and allows automation as a well established complement to manual operation.Meanwhile,some factors which influence on the morphology of filamentous microorganisms,such as agitation intensity,dissolved oxygen concentration and dilution rate,were also discussed here.Most studies on the effects of agitation intensity on fungal morphology were focused on the relation between hyphal length and specific energy dissipation rate.A shaving mechanism was proposed to explain the effects of agitation intensity on the morphology of pellets.The effects of agitation intensity and dissolved oxygen concentration on fungal morphology proved to be related.There is no common result on the effect of dilution rate on fungal morphology at present.

	Service

	Email this article
	Add to my bookshelf
	Add to citation manager
	Email Alert
	RSS
	Articles by authors

Keywords：

Received 2002-05-15; published 2002-05-15

Cite this article:

LI Xiao jing 1,YANG Kui 2,FENG Xia 2 .Morphological Analysis Methods and Main Factors Influencing Morphology of Fliamentous Microorganisms[J] Chemcial Industry and Engineering, 2002,V19(3): 233-237

[1]	METZB ,BRUIGNEW ,SUIJDAMJC .Methodforquantitativerepresentationofthemorphologyofmolds[J].BiotechnologyandBioengineering,1981,23:149162.
[2]	SUIJDAMJC ,METZB .Influenceofmechanicalvariablesuponthemorphologyoffilamentousmolds[J].BiotechnologyandBioengineering,1981,23:111-148.
[3]	ADAMSHL ,THOMASCR .Theuseofimageanalysisformorphologicalmeasurementsonfila mentousmicroorganisms[J].BiotechnologyandBioengineering,1988,32:707-712.
[4]	PACKERHL ,THOMASCR .Morphologicalmea surementsonfilamentousmicroorganismsbyfullyautomaticimageanalysis[J].BiotechnologyandBioengineering,1990,35:870-881.
[5]	COXPW ,THOMASCR .Classificationandmea surementoffungalpelletsbyautomatedimageanal ysis[J].BiotechnologyandBioengineering,1992,39(9):945-952.
[6]	JUSTENP ,PAULGC ,NIENOWAW ,etal.De pendenceofmycelialmorphologyonimpellertypeandagitationintensity[J].BiotechnologyandBio engineering,1996,52(6):672-684.
[7]	TRESKATISSK ,ORGELDINGERV ,WOLFH ,etal.Morphologicalcharacterizationoffilamentousmicroorganismsinsubmergedculturesbyon-linedigitalimageAnalysisandPatternRecognition[J].BiotechnologyandBioengineering,1997,53(2):191-201.
[8]	阳　葵,王积分,王福东,等.形态解析法用于菌体生长[J].化工学报,1999,50(4):573-576.
[9]	VENCHT -LIFSHITZSE ,MAGDASSIS ,BRAUNS .Pelletformationandcellularaggregationinstreptomycestendae[J].BiotechnologyandBio engineering,1990,35:890-896.
[10]	CUIYQ ,VANDERLANSRGJM ,LUYBENKCAM ,etal.Effectsofdissolvedoxygentensionandmechanicalforcesonfungalmorphologyinsub mergedfermentation[J].BiotechnologyandBio engineering,1998,57(4):409-419.
[11]	CUIYQ ,VANDERLANSRGAM ,LUYBENKCAM ,etal.Effectofagitationintensitiesonfungalmorphologyofsubmergedfermentation[J].Bio technologyandBioengeering,1997,55(5):715-725.
[12]	CUIYQ ,OKKERSEWJ ,VANDERLANSRGJM ,etal.Modelingandmeasurementsoffungalgrowthandmorphologyinsubmergedfermentations[J].BiotechnologyandBioengeering,1998,60(2):216-229.
[13]	KENICHIHIGASHIYAMA ,KATSUSHIMURAK MI ,HIDEOTSUJIMUA ,etal.Effectsofdissolvedoxygenonthemorphologyofanatachidonicacidproductionbymortierellaalpina1S -4[J].Bio technologyandBioengeering,1999,63(4):442-448.
[14]	MARILIYNGWIEBE ,ANTHONYPJTrinci.Dil lutionrateasadeterminantofmycelialmorphologyincontinuousculture[J].BiotechnoloygandBio engeering,1991,38:75-81.
[15]	MilesEA ,TRINCIAPJ .EffectofpHandtem pretureonmorphologyofbatchchemostatculturesofpenicilliumchrysogenum[J].TransBrMycolSoc,1983,81:193-200