Application of Natural Clarifying Agent of ZTC1+1 in Purifying Polysaccharides from Mulberry Leaves
LIN Jiang-bo,LI Shu-fen,YANG Hong-ling,JIA Dong-dong(Key Laboratory for Green Chemical Technologies of the Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China)
(Key Laboratory for Green Chemical Technologies of the Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China
Abstract This paper aims to study the effect of natural clarifying agent of ��ZTC1+1 on the purification of polysaccharides extracted from mulberry leaves.The content of polysaccharides in the extracts was determined by anthracenonesulphuric acid method and the optimum conditions were obtained by orthogonal experimentations.The results showed that the natural clarifying agent of �� ZTC1+1 can effectively extract polysaccharides from mulberry leaves,while some impurities were removed.The optimum experimental conditions were as follows: concentration ratio is 5��1 mL/g;the adding amount of natural clarifying agent((i.e) B and A) to the concentrated solution are 2% and 1% respectively;the clarifying temperature is 50 ��.Under above conditions,the retention ratio of polysaccharides was above 96%,the content of polysaccharides in the extracts was near 22% and the removal of proteins was 16%.
LIN Jiang-bo,LI Shu-fen,YANG Hong-ling,JIA Dong-dong.Application of Natural Clarifying Agent of ZTC1+1 in Purifying Polysaccharides from Mulberry Leaves[J] Chemcial Industry and Engineering, 2008,V25(1): 40-43
2008, Vol. 25 
