Optimization of preparing crude microcrystalline cellulose from okara by response surface methodology

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Abstract In this study, crude microcrystalline cellulose (CMCC) was prepared from soybean residue(okara) through combined treatment of potassium hydroxide and wet-process phosphoric acid. The crystallinity index was chosen as the response value and single factor experiments, Plackett-Burman experiments, and response surface methodology were used to optimize process conditions. The results suggested that alkali treatment temperature, acid treatment temperature, and acid treatment time had a significant impact on crystallinity index of CMCC and the optimal process conditions were identified as alkali treatment temperature 90 ℃, alkali treatment time 60 min, potassium hydroxide solution concentration 4%, wet-process phosphoric acid concentration 35%(P₂O₅), acid treatment time 60 min, and acid treatment temperature 95 ℃. The confirmatory experiment indicated the crystallinity index of CMCC was 42.89% under optimal conditions, which was only 0.12 percentage points lower than the predicted value, indicating that the optimization results obtained by the response surface optimization method are reasonable and feasible.

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	XU Lu
	SUN Chongqing
	XU Dehua
	YAN Zhengjuan
	LI Xue
	ZHONG Benhe
	WANG Xinlong

Keywords： soybean residue crude microcrystalline cellulose crystallinity index Plackett-Burman experiment response surface optimization experiment

Received 2024-02-01;

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XU Lu, SUN Chongqing, XU Dehua, YAN Zhengjuan, LI Xue, ZHONG Benhe, WANG Xinlong.Optimization of preparing crude microcrystalline cellulose from okara by response surface methodology[J] Chemcial Industry and Engineering, 2025,V42(1): 1-8

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