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Chemcial Industry and Engineering 2015, Vol. 32 Issue (4) :73-78    DOI: 10.13353/j.issn.1004.9533.2015.04.014
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Construction of An Efficient Xylose-Utilizing Saccharomyces cerevisiae Strain by Deletion of PHO13 Gene and Evolutionary Engineering
Wang Chenyu, Zang Guochang, Chen Xun
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract 

Xylose is the second most abundant sugar and the most prevalent pentose sugar found in lignocelluloses. To improve xylose utilization, significant efforts have focused on the metabolic engineering of Saccharomyces cerevisiae. In this study, efficient xylose-utilizing Saccharomyces cerevisiae strain PE, was constructed by deletion of PHO13 gene encoding para-nitrophenyl phosphatase and subjected to EMS mutagenesis followed by evolution engineering in an industrial recombinant strain, KAM-6X, which over expressed the genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase. Under aerobic condition, the specific growth rate of PE was 0.299 h-1 and 0.282 h-1 in YPX containing 50 g/L and 100 g/L xylose, which improved about 95.43% and 102.87%, respectively. And PE strain can consume 36.12 g/L xylose in 24 h and 70.25 g/L xylose in 48 h. Under oxygen-limited condition, ethanol yield reached 0.382 g/g with less glycerol production and high acetate level. It proves that PE is an efficient xlose-fermenting industrial yeast strain.

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Wang Chenyu
Zang Guochang
Chen Xun
Keywordsxylose;   PHO13;   evolutionary engineering;   Saccharomyces cerevisiae   ethanol     
Received 2013-04-23;
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Wang Chenyu, Zang Guochang, Chen Xun.Construction of An Efficient Xylose-Utilizing Saccharomyces cerevisiae Strain by Deletion of PHO13 Gene and Evolutionary Engineering[J]  Chemcial Industry and Engineering, 2015,V32(4): 73-78
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