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Chemcial Industry and Engineering 2013, Vol. 30 Issue (5) :21-25    DOI:
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Screening of a Flocculant-Producing Bacterial Strain and Study on Its Flocculating Activity
 HAO  Jian-An, YANG  Bo, JIANG  Tian-Xiang, ZHANG  Xiu-Zhi, ZHANG  Xiao-Qing, ZHANG  Ai-Jun, WANG  Jing, ZHANG  Yu-Shan
(The Institute of Seawater Desalination and Multipurpose Utilization, State Oceanic Administration, Tianjin 300192, China)

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Abstract Flocculants were commonly used agents in seawater purification. Seawater flocculants were hot spot research area. In this study, we collected samples from Bohai Gulf. Plate dilution method was applied to isolate bacteria. Improved detection method was developed to screen flocculating active strains. A bacterial strain with high flocculating activity was found. The strain was identified as Bacillus licheniformis by 16S rDNA test and biolog test. It was nominated as Bacillus licheniformis dhs-36 ultimately. According to the flocculating rate analysis of culture, supernatant and broken cell product, we found the flocculating activity of the strain was only in fermentation supernatant. Flocculating active curve showed the strain had best flocculating activity after two days. Following the stability test of flocculating activity, we found the flocculating activity of the strain was sensitive to pH. The strain could only maintain flocculating activity while pH varied from 6 to 10. But it could maintain flocculating activity while temperature varied in a wide range. The flocculating active substance was identified as protein by saccharide, protein, lipid, and nucleic acids qualitative test. High flocculating activity, convenient collecting method of flocculating active substance made this bacteria a promising material in seawater treatment.
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Articles by authors
HAO Jian-An
YANG Bo
JIANG Tian-Xiang
ZHANG Xiu-Zhi
ZHANG Xiao-Qing
ZHANG Ai-Jun
WANG Jing
ZHANG Yu-Shan
Keywordsflocculating activity   isolation and identification   active substance     
Received 2012-10-19;
Fund:

海洋公益性行业科研专项项目(201105026);国家海洋局青年海洋科学基金(2010139);中央级公益性科研院所基本科研业务费专项基金项目团队项目(K-JBYWF-2011-T02)和天津市科技兴海项目(KJXH2011-01)。

Corresponding Authors: 张雨山,电话:(022)87890659,E-mail:yushanzhang@hotmail.com。     Email: yushanzhang@hotmail.com
About author: 郝建安(1981-),男,博士,高级工程师,现从事海水净化与水再利用技术研究。
Cite this article:   
HAO Jian-An, YANG Bo, JIANG Tian-Xiang, ZHANG Xiu-Zhi, ZHANG Xiao-Qing, ZHANG Ai-Jun, WANG Jing, ZHANG Yu-Shan.Screening of a Flocculant-Producing Bacterial Strain and Study on Its Flocculating Activity[J]  Chemcial Industry and Engineering, 2013,V30(5): 21-25
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