Interaction between 4, 9di［N(2dimethylamino)ethyl］ 9Aminoacridine4Formamide and Protein

Abstract Mutual combination mechanism of DNAF with bovine serum albumin (BSA) was investigated by applying watersoluble 4,9di［N(2dimethylamino) ethyl］9aminoacridine4formamide(DNAF) probe. The corresponding thermodynamic parameters ΔH, ΔG and ΔS were calculated and through fluorescence quenching method, the number of binding sites n and apparent binding constant KA were determined. The distance of donor BSA and receptor DNAF, namely r was derived based on the theory of fluorescence resonance energy transfer. The quenching mechanism of DNAF on intrinsic fluorescence quenching of protein and the types of interaction forces were proposed.

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Keywords： 4,9di［N(2dimethylamino)ethyl］9aminoacridine4formamide protein interaction mechanism

Cite this article:

ZHANG Li-juan.Interaction between 4, 9di［N(2dimethylamino)ethyl］ 9Aminoacridine4Formamide and Protein[J] Chemcial Industry and Engineering, 2011,V28(3): 45-48