重组人白细胞介素-6包含体溶解工艺研究

摘要本文系统研究了影响大肠杆菌表达的重组人白细胞介素 6 (rhIL 6 )包含体溶解的因素 ,确定了rhIL 6包含体溶解的最佳工艺条件 ,即在 pH为 8 4的 5 0mmol/LTris HCL缓冲体系中 ,变性剂尿素和还原剂 β 基乙醇 (β ME)的浓度分别为 8mol/L和 5 0mol/L ,包含体含量为 1 0mg/mL ,在 4℃下溶解 1h。包含体溶解后蛋白含量可达到 1 3mg/mL左右 ,目标蛋白的溶解率达到 98% ,纯度为 5 0 %左右

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	吴蕾
	张瑛
	甘一如
	孙江莉
	张浩森

关键词：重组人白细胞介素-6 包含体

Abstract： The technological condition for solubilization of the recombinant human Interleuk in\|6(rhIL 6) inclusion body produced from E.coli was studied. The optimal solubilization condition was obtained:the inclusion body was solubilized at 4℃ in 50mmol/L Tris\|HCL(pH=8.4),8mol/L urea and 50 mM β ME at a concentration of 10mg/mL for about 1h. Then nearly all the target protein was solubilized and the solubilized protein concentration was solubilized and the solubilized protein concentration was about 1.3 mg/mL and the purity was up to 50%.

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Received 2000-11-15; published 2000-11-15

引用本文:

吴蕾,张瑛,甘一如,孙江莉,张浩森.重组人白细胞介素-6包含体溶解工艺研究[J]. 化学工业与工程, 2000,17(6): 321-325

WU Lei,ZHANG Ying, GAN Yi\|ru,SUN Jiang\|li, ZHANG Hao sen .A Study for Solubilization of rhIL 6 Inclusion Body Produced from E.Coli[J]. Chemcial Industry and Engineering, 2000,17(6): 321-325