肺炎克雷伯氏菌培养工艺的研究

摘要本文研究了肺炎克雷伯氏菌的培养工艺 ,得到了实验范围内最佳的培养基组成和培养条件 :葡萄糖 1% (质量分数 ,下同 )、大豆胨 1 5 %、牛肉浸膏 0 2 %、酵母粉0 2 %、蛋白胨 0 5 %、氯化钠 0 5 %、K2 HPO40 35 %、KH2 PO40 15 %。 2 %接种量 ,转速16 0r min和 2 0 %的摇瓶装填量是适宜的

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	方文
	吴蕾
	葛新
	张瑛
	甘一如

关键词：肺炎克雷伯氏菌荚膜糖蛋白培养工艺条件

Abstract： The cultivation of Klebsiella pneumoniae in flask was studied. The medium recipe suitable to Kp-9 strain growth and expression of the capsular glycoprotein was proposed. The ingredients of the recipe include glucose 1%, soya peptone 1.5%, beef extract 0.2%, yeast extract 0.2%,tryptone 0.5%,NaCl 0.5, K 2HPO 4 0.35% and KH 2PO 4 0.15%. The suitable culture conditions are 160r/min of rotate speed, 20% of filling capacity and 2% of inoculum volume.

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Received 2002-01-15; published 2002-01-15

引用本文:

方文 ,吴蕾,葛新,张瑛,甘一如.肺炎克雷伯氏菌培养工艺的研究[J]. 化学工业与工程, 2002,19(1): 6-11

FANG Wen 1,WU Lei 1,GE Xin 2,ZHANG Ying 1,GAN Yi-ru 1 .Study on Culture Technological Conditions of Klebsiella Pneumoniae[J]. Chemcial Industry and Engineering, 2002,19(1): 6-11