有机相中包衣脂肪酶催化酯化反应动力学

摘要用合成的谷氨酸十二烷基酯核糖醇对来源于Candidarugosa的脂肪酶包衣后在有机溶剂中催化月桂酸和月桂醇的酯化反应。确定了最佳反应条件 :制备包衣酶用缓冲液的最适pH为 6 8,温度 30℃ ,有机溶剂为异辛烷。酯化反应符合单底物醇抑制的Ping -PangBi-Bi机制。在最适反应条件下 ,月桂酸和月桂醇的米氏常数分别为 2 9 1mmol·L- 1和 71 4mmol·L- 1,月桂醇的抑制常数为 1 1 3mmol·L- 1,最大反应速率为 0 6 6mmol·min- 1·g- 1。动力学模型预测值与实验结果吻合良好

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	丁辉
	邢爱华
	宋宝东
	吴金川

关键词：脂肪酶表面活性剂包衣酶酯化反应有机溶剂

Abstract： The lipase from Candida rugosa was coated with synthetic didodecanyl glutamate ribitol and used to catalyze esterification of lauric acid and lauryl alcohol in organic solvents.The optimal reaction conditions were determined,which are buffer pH for lipase coating,6 8;reaction temperature,30℃;solvent,isooctane.The kinetics of the esterification reaction obeyed the Ping-Pang Bi-Bi mechanism with an inhibition by lauryl alcohol.The kinetic parameters under the optimal conditions were determined.The Michaelis constants of lauric acid and lauryl alcohol were 29 1 and 71 4mmol·L -1 ,respectively.The maximum rate was 0 66mmol·min -1 ·g -1 .The inhibition constant of lauryl alcohol was 11 3mmol·L -1 .The results predicted by the kinetic model were in good agreement with experiment.

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Received 2002-11-15; published 2002-11-15

引用本文:

丁辉,邢爱华,宋宝东,吴金川.有机相中包衣脂肪酶催化酯化反应动力学[J]. 化学工业与工程, 2002,19(6): 417-421

DING Hui,XING Ai-hua,SONG Bao-dong,WU Jin-chuan .Kinetics of Esterification Reactions Catalyzed by Surfactant-Coated Lipase in Organic Solvents[J]. Chemcial Industry and Engineering, 2002,19(6): 417-421

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