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�ؼ���� 򵰰�(IgY) ��Ƭ��(Fab) ��״��

Abstract�� Human Rotavirus(HRV) were used as antigen to immunize laying chickens and anti-HRV IgY was isolated from the yolk of eggs using two salting-out steps followed by one-gel filtration chromatography methods.The purity was over 95% by SDS-PAGE analysis.The purified IgY was digested with pepsin and the smaller antibody fragment Fab' was obtained.The purified Fab' was characterized by SDS-PAGE and MALDI MS methods,and its purity was over 99%.The results indicate that the methods designed in this paper for isolating hen antibodies(IgY and Fab') are simple and effective,the retained Fab' is over 70% of the initial activity of IgY determined by the ELISA method.

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Received 2006-07-15; published 2006-07-15

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��;�δ��;��;��;.��״��IgY��Fab��ķ��봿��[J]. ��ѧ��ҵ�빤��, 2006,23(4): 317-319

SUN Shu-qing,DUAN Chun-yan,HU Yan-tao,MENG Yan.Preparation and Purification of IgY and Fab' Against Human Rotavirus[J]. Chemcial Industry and Engineering, 2006,23(4): 317-319

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