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Abstract�� The degree of deacetylation has a great effect on the physiochemical and bioactive properties of chitosan. In this paper,the acetylation of chitosan in acetic acid-water-methonal solution was studied, the influence of reaction time and chitosan mass concentration were investigated,and the reaction conditions were optimized. The results showed that 6 h was enough for acetic anhydride to be fully reacted,no necessary to take 24 h as reported. The experimental data of gel permeation chromatography suggested there was little effect on weight average molar mass of chitosan by acetylation,but molar mass distribution of chitosan became boarder after acetylation. Taken 6 h as reaction time,chitosan with different degree of deacetylation were prepared through altering the addition amount of acetic anhydride. Steady-state fluo- rescence spectroscopy suggested that critical aggregation concentration( CAC) of chitosan increased with the decrease of chitosan degree of deacetylation.

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Received 2010-05-15; published 2010-05-15

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��ϼ;.��ͬ��ȿǾ��ǵ��Ʊ��ۼ��Ϊ�о�[J]. ��ѧ��ҵ�빤��, 2010,27(3): 205-209

WANG Li-xia .Preparation and Aggregation Behavior of Chitosan with Different Degrees of Deacetylation[J]. Chemcial Industry and Engineering, 2010,27(3): 205-209

[1]	��,��ϼ,��,��.NMR��FITR��λ�ζ��ⶨ�Ǿ��[J].��ܸ߷��ѧ��,2003,16(9):313-318
[2]	TOMIHATA K,IKADA Y.In vitro and in vivo degrada-tion of films of chitin and its deacetylated derivatives[J].Biomaterials.1997,18(7):567-575
[3]	KIANG T,WEN J,LIM H W,et al.The effect of the de-gree of chitosan deacetylation on the efficiency of gene transfection[J].Biomaterials.2004,25(22):5293-5301
[4]	CHATELET C,DAMOUR O,DOMARD A.Influence of degree of acetylation on some biological properties of ch-itosan films[J].Biomaterials.2001,22(3):261-268
[5]	AMIJI M M.Pyrene fluorescence study of chitosan self association in aqueous solution[J].Carbohydrate Poly-mers,1995,26(3):21l-213
[6]	WU C,ZHOU S Q,WANG W,A dynamic laser light-scattering study of chitosan in aqueous solution[J].Bio-polymers,1995,35(4):385-392.
[7]	ʷ��,��,��.ӫ��̽�뷨�о��Ǿ��ˮ��Һ�еľۼ��Ϊ[J].�й��ѧ��⻯ѧ,1998,16(2):161-166
[8]	���,��,��,��.�Ǿ��ϡ��Һ�еľۼ��Ϊ[J].Ӧ�û�ѧ,2004,21(2):159-163
[9]	YANAGISAWA M,KATO Y,YOSHIDA Y,et al.SEC-MALS study on aggregates of chitosan molecules in a-queous solvents:Influence of residual N-acetyl groups[J].Carbohydrate Polymers.2006,66(2):192-198
[10]	AIBA S I.Preparation of N-acetylchitooligosaccharides by hydrolysis of chitosan with chitinase followed by N-acetylation[J].Carbohydrate Research.1994,261(2):297-306
[11]	BERTH G,DAUTZENBERG H.The degree of acetyla-tion of chitosans and its effect on the chain conformation in aqueous solution[J].Carbohydrate Polymers.2002,47(1):39-51
[12]	SORLIER P,DENUZIERE A,VITON C,et al.Relation between the degree of acetylation and the electrostatic properties of chitin and chitosan[J].Biomacromole-cules.2001,2(3):765-772
[13]	��ڶ�,��,��ӷ�,��.��ȫˮ��ԿǾ��ǵ��Ʊ��[J].�Ϻ�ˮ��ѧѧ��,2000,9(4):373-375
[14]	��ϼ,�¹��,��.��һ�׵��ȷ��ⶨ�Ǿ��ǵ��[J].��:��ѧ�ֲ�,2003,39(4):195-198
[15]	BRUGNEROTTO J,DESBRIERES J,ROBERTS G,et al.Characterization of chitosan by steric exclusion chro-matography[J].Polymer.2001,42(25):9921-9927
[16]	VACHOUD L,ZYDOWICZ N,DOMARD A.Formation and characterization of a physical chitin gel[J].Carbo-hydrate Research.1997,302(3/4):169-177
[17]	WINNIK F M,WINNIK M A,TAZUKE S.Interaction of hydroxypropylcellulose with aqueous surfactants:fluores-cence probe studies and a look at pyrene-labeled poly-mer[J].The Journal of Physical Chemistry.1987,91(3):594-597