Optimization of Formulation and Preparation Parameters for Nimesulide Loaded NLC by Orthogonal Design
DU Guang-Sheng1,2, LI De-Xin2, WEI Zhen-Ping1
(1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; 2. Chemical Pharm R&D Institute, Tianjin Tasly group, Tianjin 300410, China)
Abstract：
High pressure homogenization method was used to prepare Nimesulide loaded nanostructured lipid nanoparticles (NLC). Single factor design was chosen to study the influence of solid-liquid lipid ratio, amount of lipid, amount of emulsifier, drug-lipid ratio, homogenization pressure and homogenization cycles on quality of the prepared nanoparticles. Orthogonal design was used to optimize the preparation parameters. The optimal preparation method was 3:10 of oleic acid glycerin monostearate(GMS) ratio, 1:40 of drug-lipid ratio, 1.4 g of TPGS, 90 MPa of homogenization pressure. Particle size and entrapment efficiency of NLC prepared by optimal conditions were 132.6±1.4 nm and 82.35±1.23%, respectively. The scanning electron microscope showed that the nanoparticles were of spherical shape. The in vitro release study showed that release behavior accorded with Higuchi model.
DU Guang-Sheng, LI De-Xin, WEI Zhen-Ping.Optimization of Formulation and Preparation Parameters for Nimesulide Loaded NLC by Orthogonal Design[J]. Chemcial Industry and Engineering, 2014,31(4): 46-51
2014, Vol. 31 
