反相高效液相色谱测定在乙醇酸甲酯存在下的乙醇酸含量

摘要建立了一种采用反相高效液相色谱法定量分析乙醇酸甲酯及其水解产物乙醇酸的方法。高效液相色谱仪为Agilent 1100，检测器为紫外（UV）检测器，ZORBAX SBC18(4.6 mm×150 mm,5 μm）型色谱分离柱，柱温为25 ℃，流动相为乙腈磷酸水溶液［V（乙腈）∶V（磷酸水溶液）为20∶80］，流速为0.4 mL/min，检测波长为214 nm，线性回归曲线的相关系数R﹥0.999 6。该方法的回收率为99.30%～105.96%，精密度RSD为0.3%～1.0%。结果表明，该方法简单、快速、准确，适用于乙醇酸甲酯水解体系的分析测定。

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	黄光晓
	徐艳
	赵玉军
	马新宾

关键词：反相高效液相色谱乙醇酸甲酯乙醇酸

Abstract： A method for quantification of methyl glycolate and the hydrolysis product glycolic acid using high performance liquid chromatography was developed.The apparatus type was an HP1100 series high performance liquid chromatography with VWD.Separation was performed on a ZORBAX SBC18 column（4.6 mm×150 mm，5 μm）The column temperature was 25 ℃ and the mobile phase was acetonitrile and phosphoric acid aqueous solution（20∶80，V/V）The flow rate was 0.4 mL/min with ultraviolet detection wavelength at 214 nm.A good linear regression curve was obtained with a favorable correlation coefficient（greater than 0.999 6）.The method showed high average recoveries for the samples ranged from 99.30% to 105.96% and good precision（RSD）ranged from 0.3% to 1.0%.The results showed that the method was simple，fast and accurate enough to apply to the hydrolysis system of methyl glycolate.

Keywords： RPHPLC methyl glycolate glycolic acid

Received 2011-09-27;

About author: 黄光晓（1987-），女，河北邢台人，硕士研究生。

引用本文:

黄光晓, 徐艳, 赵玉军, 马新宾.反相高效液相色谱测定在乙醇酸甲酯存在下的乙醇酸含量[J]. 化学工业与工程, 2012,29(01): 48-51

HUANG Guang-Xiao, XU Yan, ZHAO Yu-Jun, MA Xin-Bin.RP-HPLC Determination of Glycolicacid in Presence of Methyl Glycolate[J]. Chemcial Industry and Engineering, 2012,29(01): 48-51