4,9二［N(2二甲氨基)乙基］9吖啶胺4甲酰胺与蛋白质相互作用

摘要应用水溶性4,9二［N(2二甲氨基)乙基］9吖啶胺4甲酰胺（DNAF）探针，研究了DNAF与牛血清白蛋白（BSA）的相互结合作用机理。计算出相应的热力学参数ΔH、ΔG以及ΔS；通过荧光猝灭方法研究结合作用，测定出结合位点数n和表观结合常数KA；根据荧光共振能量转移理论求得供体BSA和受体DNAF的距离r；研究了DNAF对蛋白质内源荧光猝灭的猝灭机制和主要作用力类型。

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关键词： 4,9二［N(2二甲氨基)乙基］9吖啶胺4甲酰胺蛋白质相互作用机理

Abstract： Mutual combination mechanism of DNAF with bovine serum albumin (BSA) was investigated by applying watersoluble 4,9di［N(2dimethylamino) ethyl］9aminoacridine4formamide(DNAF) probe. The corresponding thermodynamic parameters ΔH, ΔG and ΔS were calculated and through fluorescence quenching method, the number of binding sites n and apparent binding constant KA were determined. The distance of donor BSA and receptor DNAF, namely r was derived based on the theory of fluorescence resonance energy transfer. The quenching mechanism of DNAF on intrinsic fluorescence quenching of protein and the types of interaction forces were proposed.

Keywords： 4,9di［N(2dimethylamino)ethyl］9aminoacridine4formamide protein interaction mechanism

引用本文:

张丽娟.4,9二［N(2二甲氨基)乙基］9吖啶胺4甲酰胺与蛋白质相互作用[J]. 化学工业与工程, 2011,28(3): 45-48

ZHANG Li-juan.Interaction between 4, 9di［N(2dimethylamino)ethyl］ 9Aminoacridine4Formamide and Protein[J]. Chemcial Industry and Engineering, 2011,28(3): 45-48