蛋白质分子在聚丙烯酰胺凝胶中扩散和分配特性的研究

摘要利用凝胶内溶质分子向缓冲溶液中扩散的方法，测定了溶菌酶、β－乳球蛋白、卵清蛋白、牛血清白蛋白和γ－球蛋白５种蛋白在聚丙烯酰胺凝胶中的有效扩散系数和分配系数，研究了单体质量浓度、交联度和蛋白分子粒径等因素的影响，讨论了聚丙烯酰胺凝胶中蛋白分子的扩散和分配机制，发现不能用Ｏｇｓｔｏｎ理论解释聚丙烯酰胺凝胶中蛋白分子的扩散和分配特性

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	徐建宽
	周志明
	何明霞
	何志敏

关键词：聚丙烯酰胺凝胶蛋白质有效扩散系数分配系数

Abstract： The effective diffusion coefficient and partition coefficient of five proteins(LysozymeLactoglobulinovalbuminbovine serum albumin and Globulin)in polyacrylamide gels were measured with the method of proteins in gels diffusing into buffer solution.The effects of mass concentrations of monomer,crosslink ratio and the size of protein were studied individually.The present results indicated that the diffusion and partitioning behavior of protein molecules could not be explained by Ogston's theory.

Keywords： polyacrylamide gel protein;effective diffusion coefficient partition coefficient

Received 1999-10-15;

引用本文:

徐建宽,周志明,何明霞,何志敏.蛋白质分子在聚丙烯酰胺凝胶中扩散和分配特性的研究[J]. 化学工业与工程, 1999,16(4): 3-8

Xu JiankuanZhou ZhimingHe MingxiaHe Zhimin .The Characteristics of the Diffusion and Partitioning of Proteins in Polyacrylamide Gels[J]. Chemcial Industry and Engineering, 1999,16(4): 3-8