液相色谱法分离纯化固相合成的胸腺素α1

摘要本文为以Fmoc(Nα-芴甲氧羰基 )固相合成法制备的胸腺素α1粗品提出了一条实验室规模的液相色谱法纯化路线 ,共分两步 :第一步采用DEAESepharoseFastFlow填料进行离子交换层析操作 ,分离除去粗品中的大部分杂质 ;第二步用制备型反相高效液相色谱 (用C1 8色谱柱 )进行最后的纯化。终产物纯度可达 95% ,生物活性符合实验指标。本文还对分离纯化路线中所用的操作条件进行了研究和优选

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	黄鹤
	万里鹏
	吴蕾
	甘一如

关键词：胸腺素α1 固相合成多肽离子交换层析反相高效液相层析

Abstract： In this paper,a purification route using the liquid chromatogram on a loboratory scale was established on thymosin α1 synthesized by the solid-phase synthetic method using Fmoc group fo protecting α-amino acid.Two steps were included in the purification route.First was the Ion-exchange chromatography (IEC) using DEAE Sepharose Fast Flow as the matrix.After this step,majority of impure peptides was removed.Second was the preparation RP-HPLC on C18 column.Then the final product's purity attained to 95% and the values of the biological activity all meet the requirements.Those operation conditions during the separation and purification process were also studied and optimized in this thesis.

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Received 2001-11-15; published 2001-11-15

引用本文:

黄鹤,万里鹏,吴蕾,甘一如.液相色谱法分离纯化固相合成的胸腺素α1[J]. 化学工业与工程, 2001,18(6): 331-335

HUANG He,WAN Li-peng,WU Lei,GAN Yi-ru .Study on the Separation and Purification Techniques of Solid-phase Synthetic Shymosin α1 by the Liquid Chromatogram[J]. Chemcial Industry and Engineering, 2001,18(6): 331-335